A rapid assay for the detection of resistance to phosphine in the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae)

Resistance to the fumigant phosphine in stored product insect pests is a global problem. Diagnosis of resistance relies on bioassay developed by FAO that involves mortality assessment after 20-h fumigation pest population at discriminating concentration gas, followed 14-day post assessment. This impractical for monitoring and early detection routine management. We utilized procedure commercial test kit rapid field populations lesser grain borer, Rhyzopertha dominica (F.). established knockdown effect either susceptible or resistant insects exposing them high phosphine. assessed relationship between adult times method 18 beetle utilizing criteria single chamber, 50% 100% groups beetles, exposed 3000 ppm also determined most effective concentrations would elicit quickest while estimating recovery from exposure. Results suggest KT100 was better than KT50 KTsingle tests. Based responses populations, we approximately min can be used as viable time distinguish populations. Higher significantly elicited quicker strongly compared These findings have potential developing robust practical R. fumigators, could incorporated management program.